Moreover, we could show that XST downregulates the expression of the fibrinogen alpha chain ( fga) gene to inhibit the coagulation cascade during the process of thrombosis in zebrafish.
The results were confirmed using a green fluorescence protein (GFP)-labeled zebrafish thrombosis model. The results indicate that XST could significantly restore heart red blood cells (RBCs) intensity of thrombotic zebrafish in a dose-dependent manner, whilst decreasing RBCs accumulation in the caudal vein. Analysis of major components in normal and abnormal XST samples was performed by high performance liquid chromatography (HPLC). Zebrafish larvae with arachidonic acid (AA)-induced thrombus were applied to evaluate anti-thrombosis effects of XST and explore the potential mechanism of XST. The aim of the present study was to establish a bioassay-based approach to evaluate the biological activity of Xuesaitong injection (XST) based on an in vivo zebrafish model.
Thus, there is a great demand to establish bioactivity-based assays to ensure the quality of CM. Quality control of Chinese medicine (CM) is mainly based on chemical testing, which sometimes shows weak correlation to pharmacological effects. 3Academy of Chinese Medical Science, Zhejiang Chinese Medical University, Hangzhou, China.2School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
1College of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.Xiangwei Ma 1, Yanyu Chen 2, Shumin Jiang 1 and Xiaoping Zhao 2,3*